Gastrorchis humblotii is endemic to Madagascar and is known from 36 collections (34 occurrences). Six of these collections (Cowan 55, s.n.1, s.n.2, s.n.3; Cribb 882, and François 18), have imprecise locality information and were not taken into account in this assessment. This species is also known from four observations recorded on iNaturalist. However, because precise GPS coordinates are not available, these observations were not considered in this assessment. Seventeen collections representing 16 occurrences, and 7 locations have been made in seven protected areas, the Montagne d’Ambre National Park (eight occurrences, one location), Manongarivo Special Reserve (one occurrence, one location), Tsaratanana Natural Integral Reserve (two occurrences, one location), Marojejy-Anjanaharibe Sud-Tsaratanana Corridor (COMATSA) (one occurrence, one location), Marojejy National Park (one occurrence, one location), Ankeniheny-Zahamena Corridor Natural Resource Reserve (two occurrences, one location), and the Andohahela National Park (one occurrence, one location). In addition to these officially protected sites, four collections representing three occurrences have also been made on the footprint of a mining project undergoing intense human pressure such as forests clearing. These occurrences should be considered as extirpated due to the forest clearing by mining activities. It is also known from nine collections (nine occurrences, eight locations) from Ankarongameloka (two occurrences, one location), Angavokely (one occurrence, one location), Mandraka (one occurrence, one location), Andramanalana (one occurrence, one location), Antsevabe (one occurrence, one location), Iakora (one occurrence, one location), Angodongodona (one occurrence, one location) and Esira (one occurrence, one location) made in unprotected forests that are subjected to selective logging, timber harvesting for small-scale subsistence and slash-and-burn practices (tavy). We estimate that four of these nine occurrences (Andrianjafy 754 in Bekora, Messmer 554 in Angodongodona, Petterson 603 near Angavokely, and Rakotovao 3158 in Andramanalana will be extirpated within the next ten years. Overall, this species is known from 30 collections (28 occurrences) with precise location information representing 25 existing and three extirpated occurrences.

The extent of occurrence (EOO) of G. humblotii is estimated to be over 138,873 km² (far exceeding the limits for Vulnerable status under criterion B1) and its minimal area of occupancy (AOO) is estimated to be 92 km² (which falls within the limits for Endangered status under the criterion B2). These 25 remaining occurrences represent a total of 15 locations (sensu IUCN 2019) with respect to the main threat, mining activities, exceeding the upper limit for Vulnerable status. Despite our inference of a loss of its habitat that will induce a strong continuous decline in EOO, AOO, habitat extent, and quality, the number of subpopulations and mature individuals in the next ten years, this widespread species, with many subpopulations encompassed within the protected areas network, will still not qualify for a threatened category by then, Gastrorchis humblotii is therefore assigned a status of Least Concern.

Gastrorchis humblotii is endemic to Madagascar where it is occurs in the former provinces of Antsiranana in the Diana, Sava Regions (Andranomilolo, Ankarongameloka, Andramanalana, Manongarivo, Marojejy, Montagne d’Ambre, Tsaratanana), of Antananarivo in the Analamanga Region (Angavokely, Mandraka), of the Fianarantsoa in Ihorombe Region (Angodongodona, Bekora), of Toamasina in the Alaotra-Mangoro, Atsinanana Regions (Ambatoharanana, Ankerana, Moramanga) and Toliara in Anosy Region (Andohahela). The species is known from the eastern and central escarpment plateau.

The population of Gastrorchis humblotii is currently thought to be decreasing since the species is collected for horticultural purposes and the habitat is threatened by forest clearing due to mining activities, and forest degradation by illegal collection for horticulture, selective logging, timber harvesting for small-scale subsistence, fire-related to Cannabis cultivation. The number of mature individuals of G. humblotii is not known and cannot be estimated as it is a terrestrial herb Growing below trees in the forest.

Gastrorchis humblotii is an epiphytic forb found on moss and lichen-covered trees or a terrestrial herb growing below trees in humid, subhumid, and montane forests at elevations of 350 to 2,000 m asl (Madagascar Catalogue 2020).

This species is threatened by mining and shifting cultivation. Nine occurrences are known from unprotected forest subjected to selective logging, timber harvesting for small-scale subsistence, collect edfor horticultural purposes, fire related to Cannabis cultivation, especially in the Tsaratanana region, and slash-and-burn practices (tavy).

The species is collected for horticultural purposes.

Gastrorchis humblotii has been collected from 17 collections (16 occurrences) in seven protected areas, the Montagne d’Ambre National Park (eight occurrences), the Manongarivo Special Reserve (one occurrence), the Tsaratanana Natural Integral Reserve (two occurrences), the Marojejy-Anjanaharibe Sud-Tsaratanana Corridor (COMATSA) (one occurrence), the Marojejy National Park (one occurrence), the Ankeniheny-Zahamena Corridor Natural Resource Reserve (two occurrences), and the Andohahela National Park (one occurrence). The species is in ex situ collections (BGCI 2021). It is included in Appendix II of CITES. Ex situ conservation is recommended (micro-propagation, seed banking, and living collections). Further awareness building is recommended for local communities and national authorities.